designed to deliver the purest
QUANTUM DOTSLuminescent inorganic nanocrystals (Q-dots). The emission wavelength is a function of the crystal size -crystals of the same chemistry can have the emission maxima in a wide range. CdTe hydrophilic Q-dots are coated with -COOH groups and can be easily used for labeling purposes forchemical and biological applications.CdSe/ZnS and ZnCdSe/ZnS (core/shell type) Q-dotsare hydrophobic and are soluble in most non-polar organic solvents.
CdTe Quantum Dots, powder, hydrophilicEasily forms colloidal solutions in water. Terminated with -COOH group.Ideal for labeling purposes. Coupling with -NH2 groups can be achieved through EDC-mediated esterefication.
General labeling procedure for proteins :-
(adopted from Wang et al. Nanoletters 2002, vol. 2, No. 8, 817-822): Reaction mixture containing 0.1 µM/mL CdTe quantum dots, 2 mg/mL protein, 1 mg/ mL sulfo-NHS (CAS# 106627-54-7), 10 mg/mL EDC (CAS# 25952-53-8) in pH 7.0 PBS buffer is prepared and stored for 2-4 h at room temperature and then stored at 4°C overnight. The precipitate (unconjugated Q-dots) if any is removed by centrifugation. The stock of ready-to-use product should be stored at 4°C. Optionally it can be dyalized on a membrane with MWCO of 12000-14000 against pH 7.0 PBS buffer and stored at 4°C. Emission wavelength may slightly shift after labeling procedure.
510 ± 5 nm λ emission maximum. Particles molar weight ca. 3200 g/mol
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520 ± 5 nm λ emission maximum. Particles molar weight ca. 16000 g/mol
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530 ± 5 nm λ emission maximum. Particles molar weight ca. 20000 g/mol
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540 ± 5 nm λ emission maximum. Particles molar weight ca. 25000 g/mol
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550 ± 5 nm λ emission maximum. Particles molar weight ca. 32000 g/mol
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560 ± 5 nm λ emission maximum. Particles molar weight ca. 55000 g/mol
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570 ± 5 nm λ emission maximum. Particles molar weight ca. 59000 g/mol
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580 ± 5 nm λ emission maximum. Particles molar weight ca. 67000 g/mol
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590 ± 5 nm λ emission maximum. Particles molar weight ca. 71000 g/mol
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600 ± 5 nm λ emission maximum. Particles molar weight ca. 76000 g/mol
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